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(24) have a short while ago raised a caveat to the assertion that ADP is surely an archaeal DNA ligase substrate by suggesting that contamination on the recombinant archaeal ligase protein preparations Together with the evidently thermostable E. coli
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). The NTase domain binds towards the damaged and intact DNA strands in the foremost groove flanking the nick and also during the minimal groove on the three′-OH aspect from the nick (supplemental Fig. S3). The OB domain binds across the small groove around the confront from the duplex at the rear of the nick.
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